Scientist, Functional Genomics, Virtual Cell Initiative
Location
United States
Posted
16 days ago
Salary
Not specified
No structured requirement data.
Job Description
Job Requirements
- PhD in Cell Biology, Molecular Biology, Genomics, or another relevant field.
- 0-3 years of post-PhD experience in academia or industry.
- Extensive experience with large-scale pooled CRISPR screening, preferably with scRNA-seq readouts (e.g. Perturb-seq, CROP-seq).
- Expertise in mammalian cell culture and ability to manage multiple cell lines simultaneously.
- Experience in multi-color flow cytometry.
- Experience with mammalian cell line engineering using transfection, lentivirus production and transduction, and other common approaches.
- Experience with different molecular biology techniques (cloning, PCR, etc).
- Demonstrated ability to work both independently and in a highly collaborative multidisciplinary environment.
- Strong project management skills with the ability to independently plan, execute, and deliver results on time.
- Excellent written and verbal communication skills.
- Hands-on experience with downstream genomics protocols, especially scRNA-seq (preferably 10X Genomics workflows).
- Experience designing and cloning large pooled CRISPR sgRNA libraries.
- Experience optimizing different CRISPR-Cas9 effector systems, such as CRISPRi and CRISPRa, including vector design and delivery.
- Experience performing FACS-based cell sorting.
- Ability to analyze large datasets using Python, R, or similar tools.
- Familiarity with different NGS technologies (Illumina, Ultima).
- Experience using automation to scale, streamline, and optimize large-scale experiments.
Benefits
- The base salary range for this position is $121,250 - $159,500.
- This position is also eligible to receive an annual discretionary bonus, with the amount dependent on individual and institute performance factors.
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